Hey,

I need help with the following question from pharmaceutical analytical chemistry!

Thank you!

You will now perform a reversed phase liquid chromatography analysis of a sample containing the analytes substance X and substance Y respectively. As a stationary phase you have a C18 column, and the mobile phase you use is an 80:20 mixture of 0.1 M phosphate buffer (pH 7) methanol. The peak belonging to the basic analyte substance X has the retention time (tR1) 19.2 minutes, and the base width (wb1) 1.0 minutes, while the second peak, belonging to the acidic analyte substance Y, has the retention time (tR2) 17.8 minutes, and the base width (wb2) 0.5 minutes. a. Calculate the resolution (Rs) of the chromatographic system. b. Is the resolution in the system good enough? Please justify your answer c. The resolution can be improved by increasing the number of theoretical bottoms (N), the selectivity factor (a) and the retention factor (k) for the analytes. Describe three changes to the experimental system that you could make to increase the number of theoretical bottoms, and thus improve the resolution. justify your answer and explain how your proposed change can help improve the resolution