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hi pleas help! cant fugure out how to calc the molarity for the unknown substance y-intercept -0.04 Lecycle With the information you have gained from

image text in transcribedhi pleas help! cant fugure out how to calc the molarity for the unknown substance
image text in transcribed
y-intercept -0.04 Lecycle With the information you have gained from the three known standards you can now determine the concentration of EDMAB in the final "unknown" cuvette. Since the molar absorptivity is an intensive property, it will be the same for EDMAB in all these solutions. Use the molar absorptivity just calculated, the pathlength, and the absorbance measured for the "unknown" cuvette to solve for the concentration (c) of EDMAB using the Beer-Lambert law (Eq. 2 on page 2). The idea is that the absorbance, A, is the y-value and the product eb is the slope while c, the concentration, is the x-value of the plot. Enter this calculated value for c of the unknown below: 62.3 M To check your math, make sure your answer makes sense. If the absorbance of the unknown sample lies between the absorbance of two of the standards, then the concentration you calculate must also lie between the two concentrations of those standards Next > 5:33 PM 1/15/2021 Data Sheet Absorption Max Wavelength (nm) 310 ecycle 100uM Absorbance 2.28 50uM Absorbance 1.12 25 M Absorbance 0.54 Unknown Absorbance 1.41 Slope 23200 y-intercept -0.04 With the information you have gained from the three known standards you can now determine the concentration of EDMAB in the final "unknown" cuvette. Since the molar absorptivity is an intensive property, it will be the same for EDMAB in all these solutions. Use the molar absorptivity just calculated, the pathlength, and Next

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