FREE Trial
Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

How to calculate the volumes for the Nuclease-free water and the RNA? Can someone show me the steps please? Thanks! Solutions: - 5XcDNA synthesis buffer

image text in transcribed

How to calculate the volumes for the Nuclease-free water and the RNA? Can someone show me the steps please? Thanks!

Solutions: - 5XcDNA synthesis buffer (20C freezer ) - 5mMdNTPMix(20C freezer ) - 400ng/1 Random hexamer primer or 500ng/ l oligo dT primer (20C freezer ) - RT enhancer (20C freezer - Verso enzyme mix (20C freezer ) - Nuclease-free water (20C freezer) Reverse transcription recipe

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

General Chemisty

General Chemisty

Authors: John E. McMurry, Robert C. Fay

2nd Edition

1269962175, 978-1269962179

More Books

Students also viewed these Chemistry questions

Question

★★★★★
Julie Brown died on May 27 of the current year. She was employed before her death at a gross salary of $4,000 per month. Her pay day was the last day of each month, and her employer did not pro rate...

Answered: 1 week ago

Question

★★★★★
Is it fair for some stores to carry Brighton products at a discount but not provide the service and ambience that the company is seeking for its products? What is the ethical issue or category in...

Answered: 1 week ago

Question

★★★★★
What is something that gives you hope? Fun Get to Know You Questions

Answered: 1 week ago

Question

★★★★★
Polzin Company had sales in 2010 of $1,500,000 on 60,000 units. Variable costs totaled $840,000, and fixed costs totaled $500,000. A new raw material is available that will decrease the variable...

Answered: 1 week ago

Question

★★★★★
How to calculate the volumes for the Nuclease-free water and the RNA? Can someone show me the steps please? Thanks! Solutions: - 5XcDNA synthesis buffer (20C freezer ) - 5mMdNTPMix(20C freezer ) -...

Answered: 1 week ago

Question

★★★★★
Question 9 (1 point) You are owner of a Grocery shop and you are reviewing the sales of product A, below is the frequency distribution of the number of sales made in the year 2018. Number of Sales of...

Answered: 1 week ago

Question

★★★★★
Original advertising strategy: Q1: [50,50,20,20,60] Q2: [10,10,30,60,80] Q3: [30,30,30,50,100] Q4: [50,90,20,20,10] Calculate the standard deviation for each quarter and variances After the new...

Answered: 1 week ago

Question

★★★★★
End 1 Pass End 2 End End Healy, 2017 7. Consider the two versions of the centipede game below. Note that the only thing that changes between the two games are the payoffs. CENT-LO Treatment: 1 Pass 2...

Answered: 1 week ago

Question

★★★★★
In this problem, we numerically calculate a posterior distribution. Suppose the stimulus distribution ps ( s ) is Gaussian with mean 2 0 and standard deviation 4 . The measurement distribution px | s...

Answered: 1 week ago

Question

★★★★★
how much would the addition of 1 hour of wiring capacity increase total profit?

Answered: 1 week ago

Question

★★★★★
Provide the answer to the following Questions. I just want to cross reference my answers with someone who has mastered Database and Design Development! It would be greatly appreciated. Thank you so...

Answered: 1 week ago

Question

★★★★★
2. Say that there are 2000 cheetahs in all of Tanzania. After looking up the area of this country, find all values of C and C for which the areal detection fraction estimator gives this value...

Answered: 1 week ago

Question

★★★★★
4. Construct declarative (active voice) phrases. In particular, place the actions verb as close as possible to the beginning of the phrase, e.g. move 400 elephants instead of 400 elephants are moved.

Answered: 1 week ago

Question

★★★★★
5. Use the word reserve instead of preserve when referring to wildlife reserves (preserves) and/or wildlife sanctuaries. See, for example, the website www.game-reserve.com.

Answered: 1 week ago

Previous Question Next Question