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How would you prepare 25 ml of a 200 mM solution of HEPES (Mw = 238 g/mol) pH 7.5? Please, show the calculation. a) Add
How would you prepare 25 ml of a 200 mM solution of HEPES (Mw = 238 g/mol) pH 7.5? Please, show the calculation. a) Add 47.6 grams HEPES to ~20 ml of water, pH to 7.5, bring up to 25 ml. b) Add 1.19 grams HEPES to 25 ml of water, pH to 7.5. c) Add 47.6 grams HEPES to 25 ml of water, pH to 7.5. d) Add 1.19 grams HEPES to ~20 ml of water, pH to 7.5, bring up to 25 ml. e) Add 5.95 grams HEPES to ~20 ml of water, pH to 7.5, bring up to 25 ml.
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