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HPLC Determination of a ternary mixture. You are provided with a typical chromatogram resulted from separation of a certain ternary mixture ( B , C

HPLC Determination of a ternary mixture.
You are provided with a typical chromatogram resulted from separation of a certain ternary mixture (B,C,D) using C18 column of a length 15cm using mobile phase (70% acetonitrile and 30% water) I- Use this chromatogram to obtain values of the following required parameters :
Where;
(a) Solvent front (mobile phase).
\table[[Sequired Parameter,],[1- NTP(c),],[2- NTP(d),],[3- HETP(b),],[4- HETP(c),],[5- K'(c),],[6- K'(d),],[7- Resolution factor for c and d,],[8- Selectivity factor for b and c,],[9- Velocity of solute c,],[10- Identify the most polar compound,]]
II-Apply the following regression equations to determine the concentrations of compound d and c using the peak height:
1- Peak Height (d)=0.05+0.2C
2- Peak Height ?(c)=0.025+0.16C
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