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HPLC Determination of a ternary mixture. You are provided with a typical chromatogram resulted from separation of a certain ternary mixture ( B , C
HPLC Determination of a ternary mixture.
You are provided with a typical chromatogram resulted from separation of a certain ternary mixture BCD using column of a length using mobile phase acetonitrile and water I Use this chromatogram to obtain values of the following required parameters :
Where;
a Solvent front mobile phase
tableSequired Parameter, NTPc NTPd HETPb HETPc Kc Kd Resolution factor for c and d Selectivity factor for b and c Velocity of solute c Identify the most polar compound,
IIApply the following regression equations to determine the concentrations of compound and using the peak height:
Peak Height d
Peak Height
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