I am reposting one more time becaue I am getting close, but it is still not working quite right. I have the following dataframe I want to make a grouped bar graph from.

library(ggplot2)

# Create dataframe

df

# Plot variables

ggplot(data = df, aes(x = nucleotide)) + geom_col(aes(y = first.exon, fill = "first.exon"), position = "dodge") + geom_col(aes(y = internal.exon, fill = "internal.exon"), position = "dodge") + geom_col(aes(y = last.exon, fill = "last.exon"), position = "dodge") + scale_fill_manual(values = c("first.exon" = "light blue", "internal.exon" = "light green", "last.exon" = "pink")) + labs(title = "Number of Nucleotides in Each Exon", subtitle = "Grouped barplot using ggplot2", x = "Nucleotide", y = "Counts", fill = "") + theme_minimal()

This should put the nucleotides as the groups on the x-axis, and the exons as the subgroups and the frequency of bases on the y axis. it is working mostly, except it is still a stacked barplot instead of a grouped bar plot. I want all three bars in each subgroup beside each other along the x-axis. below is the result I am getting, which is not what I want,

This is with the current code I want it to look like this, but with the colors and titles in the current version, this is just as an example of format Nucleotides in Sequence