i) Genome mining involves a combination of genomics, bioinformatics, molecular biology and analytical chemistry. Briefly outline what takes place at each of these steps in the process. (4 marks)

ii) What are the general features of a pleiotropic approach to activating a silent biosynthetic gene cluster (BGC)? Briefly outline the main advantage and the main disadvantage of such approaches. Give one example of a pleiotropic approach that can be used to activate silent BGCs. (4 marks)