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I need question c only estion 3 continued (c) Answer ALL parts (i) The mobile phase strength increases as the solvent becomes less polar in
I need question c only
estion 3 continued (c) Answer ALL parts (i) The mobile phase strength increases as the solvent becomes less polar in reversed-phase chromatography. Explain the reason for this. (1 mark) (ii) "The mobile phase strength increases as solvent becomes more polar in normal-phase chromatography." Is this statement true? Explain the reason for your answer. (I mark) (iii) Give two differences between adsorption chromatography and partition chromatography. (4 marks) (d) Answer ALL parts A 30cm long C18 column with a plate height of 0.78mm was used for an LC-MS experiment. (i) Calculate the number of plates N for this column, and for a shorter, 150mm, column. (3 marks) Question 3 continued on next page
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