I. Pipette Calibration 1. Weigh a dry 100mL graduated beaker. 2. Pipette into the beaker 5mL of water, re-weigh. 3. Repeat Step above 2 times until a total of 15.00mL of water is contained in the beaker. 4. From each weighing, subtract the previous weighing to determine the mass of each 5mL aliquot of water. Use the mass and appropriate density (check temperature of the water used for calibration) to calculate the volume delivered by the pipette, which should be 5.1 mL or less. If your pipette volume seems to be outside this range you may need some help with pipetting technique. I. Pipette Calibration (Water Temp ) I. Pipette Calibration 1. Weigh a dry 100mL graduated beaker. 2. Pipette into the beaker 5mL of water, re-weigh. 3. Repeat Step above 2 times until a total of 15.00mL of water is contained in the beaker. 4. From each weighing, subtract the previous weighing to determine the mass of each 5mL aliquot of water. Use the mass and appropriate density (check temperature of the water used for calibration) to calculate the volume delivered by the pipette, which should be 5.1 mL or less. If your pipette volume seems to be outside this range you may need some help with pipetting technique. I. Pipette Calibration (Water Temp )