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Background (Relates to question 1,2, and 3): After graduation from Butler you decided to pursue a career in forensic science. After going on in school and completing a masters in forensics at Michigan State University, you are hired by the Indiana State Forensics lab as their analytical chemist. Your lab is equipped with the following instruments: A. Thermo Q-Exactive LC/MS, which is a quadrupole-orbitrap hybrid instrument with a liquid phase chromatography inlet and both electrospray and atmospheric pressure chemical ionization (APCI) sources. - Cost $450,000 B. An Agilent gas chromatography / mass selective detector (GC/MSD) with a quadrupole mass analyzer, a GC inlet, and both electron ionization (El) and chemical ionization (methane CI) modes. This instrument is also configured with the full Wiley (El) library of pesticides and drugs. - Cost $80,000 C. A Bruker Ultraflex Matrix-Assisted Time-of-Flight (MALDI/TOF) instrument capable of MALDI ionization capable of linear, reflectron, and MS/MS mass analysis using MALDI ionization. - Cost $400,000 D. A Sciex 5500 triple-quadrupole (QQQ) with a Shimadzu ultra performance liquid chromatography (UPLC) inlet and both electrospray and atmospheric pressure chemical ionization (APCI) sources. Cost $350,000 Your first week on the job, you are called in to support a team investigating the death of a homeless person under suspicious circumstances. The body arrives in the morgue and you are asked to try to determine the cause of death. You are sent blood, plasma, and urine samples for analysis. Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 ak in the blood extract from the deceased (assur 2000.00 343.0732 |345.0702 163.03902 285.03120 149.02348 217.10724 346.0736 LL 140 160 180 200 220 240 260 280 300 320 340 360 380 343.0732 345.0702 . |346.0736 360.0997 365.0551 50.00-370.00 1285,03157 163,03906 217.04176 249.05491 181.04162 121.0285 191.03403 95.04950 257,03663 153,06996 190 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 260 290 3 MS? 285.03157 Reset Zoom m/z 343.07367 HCD 45: IW : MS2 270 280 290 300 310 320 330 340 350 Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 ak in the blood extract from the deceased (assur 2000.00 343.0732 |345.0702 163.03902 285.03120 149.02348 217.10724 346.0736 LL 140 160 180 200 220 240 260 280 300 320 340 360 380 |343.0732 345.0702 |346.0736 360.0997 365.0551 50.00-370.00 285,03157 163.03906 217.04176 249.05491 181.04162 121.0285 191.03403 95.04950 257.03663 153,06996 190 100 110 120 130 140 150 160 170 180 200 210 220 230 240 250 260 270 290 MS? 285.03157 Reset Zoom m/z 343.07367 HCD 45: IW : MS2 270 280 290 300 310 320 330 340 350 Background (Relates to question 1,2, and 3): After graduation from Butler you decided to pursue a career in forensic science. After going on in school and completing a masters in forensics at Michigan State University, you are hired by the Indiana State Forensics lab as their analytical chemist. Your lab is equipped with the following instruments: A. Thermo Q-Exactive LC/MS, which is a quadrupole-orbitrap hybrid instrument with a liquid phase chromatography inlet and both electrospray and atmospheric pressure chemical ionization (APCI) sources. - Cost $450,000 B. An Agilent gas chromatography / mass selective detector (GC/MSD) with a quadrupole mass analyzer, a GC inlet, and both electron ionization (El) and chemical ionization (methane CI) modes. This instrument is also configured with the full Wiley (El) library of pesticides and drugs. - Cost $80,000 C. A Bruker Ultraflex Matrix-Assisted Time-of-Flight (MALDI/TOF) instrument capable of MALDI ionization capable of linear, reflectron, and MS/MS mass analysis using MALDI ionization. - Cost $400,000 D. A Sciex 5500 triple-quadrupole (QQQ) with a Shimadzu ultra performance liquid chromatography (UPLC) inlet and both electrospray and atmospheric pressure chemical ionization (APCI) sources. Cost $350,000 Your first week on the job, you are called in to support a team investigating the death of a homeless person under suspicious circumstances. The body arrives in the morgue and you are asked to try to determine the cause of death. You are sent blood, plasma, and urine samples for analysis. Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 ak in the blood extract from the deceased (assur 2000.00 343.0732 |345.0702 163.03902 285.03120 149.02348 217.10724 346.0736 LL 140 160 180 200 220 240 260 280 300 320 340 360 380 343.0732 345.0702 . |346.0736 360.0997 365.0551 50.00-370.00 1285,03157 163,03906 217.04176 249.05491 181.04162 121.0285 191.03403 95.04950 257,03663 153,06996 190 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 260 290 3 MS? 285.03157 Reset Zoom m/z 343.07367 HCD 45: IW : MS2 270 280 290 300 310 320 330 340 350 Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 Question 2. You are able to obtain accurate MS and MS/MS electrospray spectra on the unknown. Apply the approach we discussed in the 'interpretation of mass spectra' lecture to identify the unknown. Explain in detail how you worked through each step of the Interpretation: A General Protocol procedure to justify your assignment of the unknown using the spectra shown below. Unknown peak in the blood extract from the deceased (assumed poisoned) homeless person. 50 points Full scan MS Full scan MS - expanded view of molecular ion region 1 MS/MS of m/2 343 ak in the blood extract from the deceased (assur 2000.00 343.0732 |345.0702 163.03902 285.03120 149.02348 217.10724 346.0736 LL 140 160 180 200 220 240 260 280 300 320 340 360 380 |343.0732 345.0702 |346.0736 360.0997 365.0551 50.00-370.00 285,03157 163.03906 217.04176 249.05491 181.04162 121.0285 191.03403 95.04950 257.03663 153,06996 190 100 110 120 130 140 150 160 170 180 200 210 220 230 240 250 260 270 290 MS? 285.03157 Reset Zoom m/z 343.07367 HCD 45: IW : MS2 270 280 290 300 310 320 330 340 350