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Identify the data analysis methods used and explain why the methods are appropriate based on the research question. Discuss the potential weaknesses of the data

Identify the data analysis methods used and explain why the methods are appropriate based on the research question. Discuss the potential weaknesses of the data analysis methods used. Support your discussion with examples from the study. Describe the key demographics of the population sampled and identify the inclusion and exclusion criteria for participants. Key Findings: State the results of the research, describing how the researchers determined whether the results are statistically significant. Describe the differences in the results between the groups in the study and support your description with examples from the study. Data source The data source was the CCOV's Victorian Suicide Register (VSR). In Victoria, all deaths from suspected nonnatural causes must be reported to the CCOV for investigation. If at any stage of investigation, the Coroner and/or VSR coders determine the circumstances of a death are consistent with suicide, the death is entered into the VSR. The VSR entries are reviewed and updated as coroners' investigations progress, with deaths added and removed as necessary to ensure it reflects current knowledge. Cases of death by intentional selfharm but not found to be suicide are not included. Most information is directly coded from a review of coronial records, which include the police report of death to the coroner, autopsy and toxicology reports and coronial brief (which may include records from treating health professionals; statements from family, friends, witnesses and employers; and scene photography). The VSR contains data for every Victorian suicide reported between 1 January 2000 and the present. For each case, there are over 150 coded and freetext data fields that are recorded. A data dictionary guides coding. Data includes age and sex of the deceased, the suicide method, location of fatal incident, location of usual residence, and date of death report. An enhanced dataset includes sociodemographic information about sexuality, place of birth, relationship status and employment status; history of physical and mental ill health; identified interpersonal and contextual stressors; and contacts with health and other services before death. Coding data sources and associated quality processes are described in detail elsewhere.23 Case identification Cases included are those on the VSR occurring in the 10 years between 1 January 2006 and 31 December 2015, where the deceased was aged 10-19 years inclusive. Data compilation For each relevant death the full VSR record was extracted, including all core and enhanced data. The data was compiled to produce binary variables pertaining to: sex; age (10-16 or 17-19); location of usual residence (Metropolitan Melbourne or regional Victoria); country of birth (Australia or other); evidence that the deceased identified as lesbian, gay, bisexual, transgender, queer and/or intersex (LGBTQI); relationship status; diagnosed mental illness; history of suicide attempts and/or selfharm; occupation; expressions of intent to suicide; suicide method. Additionally, evidence was recorded regarding the presence of the following stressors: exposure to abuse; family/partner/peer conflict; exposure to bullying; partner separation; sexuality; substance misuse; exposure to previous suicide of another person; and postmortem presence of alcohol and/or illegal drugs in toxicology. For the purposes of this study, certain definitions in the data dictionary are important, which are detailed below. Intent: Verbal or written indicators as to presence of expressions of suicidality, both around the time of death and historically. Previous suicide attempt: Records or reports in which the deceased had engaged in selfinjury behaviour/s with any wish, expectation or probability of death. Previous selfharm: Previous selfinjury behaviour/s with no evidence to indicate these were suicide attempts. Stressors: Generally restricted to the previous six months. Stressors relevant to this study are detailed as follows. Interpersonal stressors Partner separation Family/partner violence Family/partner/peer conflict (Conflict is defined as "a situation in which interdependent people express (manifest or latent) differences in satisfying their individual needs and interests, and they experience interference from each other in accomplishing these goals".24 There is evidence that this conflict caused stress for the deceased.) Abuse The perpetrator or victim of physical, sexual, psychological, emotional, financial, legal, civic, or systemic abuse; constraints and restrictive practices; physical, passive or emotional neglect; wilful deprivation. Bullying The perpetrator or victim of repeated and intentional hurt or harm to another person or group of people who feel helpless to respond. Statistical analysis Descriptive and inferential statistics were calculated using the Stata software version 14 (StataCorp, College Station, Texas, USA). Data were summarised using frequency tables, presenting subject counts and percentages. Differences in proportions between groups (younger versus older and male versus female adolescents) were compared using chi-square tests for equal proportions or Fisher's exact tests where numbers were small. Completed suicide rates according to age and sex were estimated using Victorian population data for adolescents aged 13-19 years collected by the Australian Bureau of Statistics.21 The 95% confidence intervals (95%CI) were determined with the exact binomial distribution. All calculated pvalues were twosided and p< 0.05 indicated statistical significance. Summary data by age and sex During the 10-year period to 31 December 2015, there were 273 completed suicides in the 10-19 year age group. There were no suicide deaths aged 10-12 years, hence younger and older groups were dichotomised using the cut-off points 13-16 years and 17-19 years. There were 102 (37%) suicide deaths in the younger group and 171 (63%) in the older group. Males comprised 184 cases (67%) and females 89 (33%). Female cases comprised a larger proportion of the younger compared to older group (p=0.039), although males outnumbered females overall. Suicide counts by age are presented in Figure 1. Suicide counts by age and sex. Suicide rates are presented in Figure 2. The overall suicide rate for adolescents was 5.58 per 100,000 (95%CI: 4.94-6.29) population aged 13-19 years. The overall rates for males and females were 7.33 (95%CI: 6.31-8.47) and 3.74 (95%CI: 3.0-4.6) per 100,000 persons aged 13-19 years, respectively. The suicide rates per 100,000 persons were 3.76 (95%CI: 3.07-4.56) for younger adolescents and 7.85 (95% CI: 6.72-9.12) for older adolescents. The suicide rate for younger males (aged 13-16 years) was 4.38 (95%CI: 3.35-5.63) and for younger females was 3.1 (95%CI: 2.23-4.21) per 100,000 persons. The suicide rate for males increased from younger to older adolescence, with the suicide rate for older males (aged 17-19 years) being 11.01 (95%CI: 9.15-13.14). Suicide rates for females did not differ as much between age groups, with the rate for older females being 4.53 (95%CI: 3.34-6.0) per 100,000 persons. Suicide rates by age and sex. Overall characteristics Forty per cent of cases had a diagnosed mental illness, with no significant difference between age groups or sex. The percentage of subjects with at least one previous suicide attempt was significantly higher in those with a diagnosed mental illness (57%), compared to those without (22%, p<0.0001). Intent was indicated in almost half of all cases. The most common methods of suicide in decreasing order were hanging; train; jump from height; and firearm. Eightyseven per cent of cases had experienced at least one significant stressor preceding their death. Characteristics by age group: younger (13-16 years) vs. older (17-19 years) adolescents There was a relatively higher prevalence of previous selfharm in younger adolescents. The majority of cases under 16 years of age were students, with a higher proportion of older adolescents classified as not in formal education, employment or training (NEET). Hanging was a relatively more common method of suicide in younger compared to older adolescents. Younger adolescents who died from suicide were more likely to have been exposed to the stressors of abuse, peer conflict and bullying. Alcohol was found in postmortem toxicology in a higher proportion of older adolescents. Characteristics by sex: male vs. female adolescents Females were more likely to have demonstrated previous deliberate selfharm or a suicide attempt. Females were more likely to be students, whereas males were more likely to be in employment. Hanging was a relatively more common method of suicide in females, while firearm was utilised by males only. Females were more likely to have been exposed to abuse, peer conflict, bullying and partner conflict. Discussion This study reveals key differences in completed suicide recorded in younger compared to older adolescents, and male compared to female adolescents. Previous studies have demonstrated that young people who die from suicide are significantly more likely than the general population to have a serious mental health illness.2,3,25 This is consistent with our study, in which 40% of adolescents had a formally diagnosed mental illness, which is much higher than the reported 14% in the general Australian population.26,27 However, our study did not reflect previous adolescent suicide studies that demonstrated the prevalence of diagnosed mental illness increasing with age.7,11 The lack of difference by age group in our study may be because there was actually a high rate of mental illness (41%) in the younger adolescent group. This could suggest that adolescents who are diagnosed at a younger age with mental illness may be at increased risk for suicide. The lifetime prevalence of deliberate selfharm in adolescents has been estimated at 10-18%, with higher rates in females compared to males.27-30 Our study demonstrated that adolescents who died from suicide had higher rates of previous deliberate selfharm compared to the general population. Our study's results were consistent with previous studies demonstrating that male adolescents are more likely to die by suicide than females.2,3,31 There is a 'gender paradox' of suicide, in which suicidal behaviour and deliberate selfharm is more common in females, but completed suicide is more common in males.2 The results of our study appear to be in line with this 'gender paradox'. Known associations and risk factors for both selfharm and suicide in adolescents are similar and often overlap. These factors include low socioeconomic status, restricted educational achievement, parent separation, adverse childhood experiences, interpersonal difficulties, mental disorders, and drug and alcohol misuse.2,3,31,34 Deliberate selfharm in adolescents can be a gateway to subsequent suicidal behaviour.33 In comparison to studies in the general population, which demonstrate the prevalence of deliberate selfharm is higher in older compared to younger adolescents,27,28 our study showed that previous selfharm was relatively more prevalent in younger adolescents who died by suicide. This finding is consistent with one UK study32 and warrants further analysis, as it suggests that younger adolescents with selfharming behaviours may be at increased risk of subsequent suicide. Adolescent presentations to hospital after selfharm often involve disclosure of suicidal intent,35 and are a means for flagging increased risk for subsequent engagement with mental health professionals. In 2016, 5.1% of Australians aged 15-19 years were classified as NEET.37 Our study showed rates of NEET were much higher in adolescents who died by suicide, with a prevalence four times higher for older adolescents, and three times higher overall. Other studies have shown even higher rates of disengagement in education or employment, with up to one in five schoolaged children who completed suicide not attending school at the time of their death, and onethird of adolescents being unemployed.38-40 In line with our hypothesis, this may suggest that participation in education, training or employment may be a protective factor, and conversely that adolescents classified as NEET may be at increased risk for suicide. It is recognised that a lack of formal educational qualification is a risk for suicide.25 Unemployment and disengagement with education are likely to have detrimental effects on mental health and emotional wellbeing. The reasons why engagement in education and training or employment are likely to be important protective factors needs to be further explored. Consistent with our results, previous studies have demonstrated that hanging is the most frequent method in adolescent suicides, with this method decreasing with age.7,15,43 Previous studies have demonstrated inconsistent findings regarding evidence of intent in adolescent suicide. One psychological autopsy found the majority of young people who die from suicide communicate their intent, but not usually on the day of death.41 Other studies describe younger adolescents who died from suicide as being more likely to have demonstrated suicidal behaviour or ideation than the general population,15,42 whereas others have found younger adolescents are less likely to communicate suicidal intent.9,15 Another study showed that approximately onethird communicated suicidal intent in the preceding 12 months, with no observable difference between age groups.7 In our study, intent (both implicit and explicit) was communicated in almost half of all cases, with no significant difference between age or sex. The reasons for such differences between studies may be due to the definition of intent - particularly implicit intent - being somewhat subjective, and parameters for timing (e.g. previous 24 hours compared to previous month) may have differed between studies. Compared to the general population, young people who die from suicide are more likely to have had exposure to stressors, both chronic and acute.3,7,31,42,44 These stressors are multiple and varied and include interpersonal conflict, relationship problems, bullying and abuse. In our study, peer conflict and bullying was seen at higher rates in both younger and female adolescents. There is strong evidence highlighting bullying as a risk factor for suicidal behaviour in adolescents,45-49 with a particular impact on female suicide.47 However, to our knowledge, no previous studies have specifically demonstrated this concerning relationship between increased incidence of bullying in younger adolescent suicide. Our finding of almost six in 10 young females who died by suicide being exposed to abuse also warrants attention. Compared to the general adolescent population, children and adolescents who complete suicide are significantly more likely to have experienced physical, sexual or emotional abuse.42,50,51 Abuse in all its forms is harmful and potentially amenable to reduction. This study may add weight to efforts that contribute to abuse prevention and postabuse supports. Relationship breakdown, interpersonal loss and rejection are also common precipitants to youth suicide,41 and the prevalence of relationship problems in suicide has been shown to increase with age.7,11 Our study did not observe this increase by age, but this may be because there was actually a relatively high proportion (29%) of younger adolescents exposed to partner conflict. This may suggest an association between partner conflict in younger adolescents and risk of suicide. In Australia, the legal age for purchase and consumption of alcohol is 18 years. Alcohol abuse is less common in younger children compared to adolescents who complete suicide,14,52 and is consumed less frequently prior to death compared to older adolescents.9,15 Our study may reflect this trend, with younger adolescents having alcohol on postmortem toxicology at almost half the incidence of older adolescents. Limitations The VSR is a comprehensive and detailed database. However, data quality is affected by the potential for variability obtained from external sources. For example, data obtained from family reports to police or the court may be inconsistently obtained due to the emotional complexities of bereavement, with cultural and religious overlay. Although we have referenced studies demonstrating prevalence of possible suicide associations and risk factors in the community (including NEET, diagnosed mental health illness, deliberate selfharm, exposure to abuse), this study did not have a community control group. This must be taken into account when drawing conclusions from our data. The findings of this study may be subject to survivor bias; that is, the findings are not applicable if the survivor population is different from nonsurvivor population. Hence, caution is required when determining relevance to adolescents with selfharming or suicidal behaviours, as those who survive suicide attempts may have different characteristics from those who do not. Although stressors were coded for the preceding six months, we were not able to accurately capture the timing of a stressor, only its presence or absence. Our study recorded exposure to the stressors of abuse and bullying, but did not differentiate between perpetrator and victim status. Elucidating this difference would be especially helpful for prevention and intervention strategies

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