If a Bradford protein assay were conducted for a solution that, in addition to containing an unknown amount of protein, also happened to contain a substantial amount of a non-protein substance that absorbs 595 nm light, the spectrophotometric analysis would yield absorbance values that are of the protein content. O lower than expected, leading to an overestimate O lower than expected, leading to an underestimate O higher than expected, leading to an overestimate O higher than expected, leading to an underestimate