Question
Imagine that you perform a gradient scouting run with an HPLC. The mobile phase composition goes from 20% organic to 80% organic over the
Imagine that you perform a gradient scouting run with an HPLC. The mobile phase composition goes from 20% organic to 80% organic over the course of 15 minutes. Your column is 200 mm long with a 3 mm internal diameter. What flow rate should you use to get a gradient retention factor of 8 for your analyte? Express your answer in mL/min to 2 decimal places.
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