Immunoglobulin IgG in CHO cell fed$-$batch culture with the addition of substrate $($glucose$).$ The initial culture volume at quasi$-$steady state is V $=250$ L and glucose$-$containing feed is added with a flow rate of F $=40$ L$/$h$.$ Glucose concentration in the feed stream is at S$0=250$ g$/$L$.$ The initial biomass is $20$ g$/$L$.$ If mu max $=0\mathrm{.}3$ h$-1,$ Ks $=0\mathrm{.}7$ g$/$L$,$ and Y X$/$S $=0\mathrm{.}45$ g cells$/$g glucose. The quasi$-$steady state assumption applies to the biomass and glucose. At $24$ hours: $($a$)$ What is the culture volume? $($b$)$ What is the concentration of glucose $($assuming quasi$-$steady state$)?($c$)$ Calculate the concentration and total amount of CHO cells at that time