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In a biology experiment studying the relation between substrate concentration [S] and reaction rate in an enzyme-mediated reaction, the data in the following table were
In a biology experiment studying the relation between substrate concentration [S] and reaction rate in an enzyme-mediated reaction, the data in the following table were obtained. [S] 0.038 0.194 0.425 0.626 1.253 2.500 3.740 rate 0.050 0.127 0.094 0.2122 0.2729 0.2665 0.3317 It is desired to find a curve (model function) of the form of saturation-growth-rate equation : Vmax[S] rate = Km + [S] that best fits the data in the least-squares sense, with the parameters Vmax and KM to be determined. Hint: you can linearize the saturation-growth-rate equation. The following solution used the Gauss-Newton method, not the same as the question asked. 0. 2 1 O a.as Figure 1: Curve model
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