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In a gas chromatography experiment, a standard solution containing 25ppm of diacetyl, represented by X, and 0.05ppm of internal standard 2,3-hexanedione, represented by S, give

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In a gas chromatography experiment, a standard solution containing 25ppm of diacetyl, represented by X, and 0.05ppm of internal standard 2,3-hexanedione, represented by S, give peak areas of Ax=330 and As=59 (areas in arbitrary units), respectively. The response factor or response ratio (F) can be calculated using the following equation and rearranging the equation to solve for F : [X]Ax=F([S]As) F must be determined by measuring known concentrations of both diacetyl and 2,3-hexanedione first, before you can use this equation to determine an unknown concentration of diacetyl. If a calibration curve is created, one must determine the signal from both the analyte and internal standard and plot the relative signal vs. the concentration of the analyte standard, as shown in the data in Table 2. Table 2. Signal collected for 2,3-butanedione samples spiked with a 2,3-hexanedione internal standard. Q14. Calculate the signal ratio for each standard and fill in the results in the empty columns of Table 2 . Calculate F for the 25 and 50 ppm standards. Are the F values similar/different

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