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In gluconeogenesis, (i) GAPDH catalyzes the reverse reaction relative to glycolysis: conversion of 1,3-BPG to GAP. What is the mechanism of this reaction? (ii) 1,3-BPG

In gluconeogenesis, (i) GAPDH catalyzes the reverse reaction relative to glycolysis: conversion of 1,3-BPG to GAP. What is the mechanism of this reaction? (ii) 1,3-BPG harbors a high- energy acyl-phosphate bond, which is broken upon its GAPDH-catalyzed conversion to GAP. Yet, the standard free energy change associated with this reaction is very small. Why?

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