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In lay-man terms or english and as clear as you can please thoroughly explain each figure, diagram and what is going on and do so
In lay-man terms or english and as clear as you can please thoroughly explain each figure, diagram and what is going on and do so in relation to the paper which I put in the reference. Use the paper to explain the figures.
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REFERENCE needed ------ this figures are part and came from a research paper titled Topic ( A programmable dual guided DNA endonuclease in Adaptive Bacterial Immunity by Jinek et al)
A B D protospacer 2 mismatch 20 15 10 5 PAM Target DNA WT 5' -AACATAACTCAATTTGTAAAAAA-GGGT-3' protospacer 2 uncut WT 22 10 5 4 3 protospacer 2 oligonucleotide DNA oligonucleotide DNA 3 ' -TTGTATTGAGTTAAACATTTTTT-CCCA-5' nicked- 1-42 1-42 1-42 linear- 5 ' -AUAACUCAAUUUGUAAAAAA-GUUUU. . .-3 ' CrRNA-sp2 crRNA-sp2 - supercoiled- 15-44 1-42 23-44 1-42 23-48 1-42 crRNA-sp2 15-89 1-42 4-89 1-42 15-53 1-42 23-89 1-42 23-53 1-42 4-89 1-36 4-89 11-42 4-89 1-32 mismatched targets tracrRNA * 15-36 1-42 1-26 tracrRNA 4-89 WT 22 5' -AGCATAACTCAATTTGTAAAAAA-3' Cas9 - + * ACRISPR Cas9 + 5 2x105 10 5' -AACATAACTCAATCTGTAAAAAA-3' 7 5' -AACATAACTCAATTTGAAAAAAA-3' CFU/pg of DNA 1x105 6 5' -AACATAACTCAATTTGTTAAAAA-3 ' 50 nt - 50 nt - 5 5' -AACATAACTCAATTTGTATAAAA-3' NT 22 10 4 5 ' -AACATAACTCAATTTGTAATAAA-3' backbone protospacer 2 target plasmid 3 5 ' -AACATAACTCAATTTGTAAATAA-3 23 nt - 23 nt - E 20 15 PAM Target DNA protospacer 2 mismatch WT 5' -AACATAACTCAATTTGTAAAAAA-TGGC-3 ' 3 ' -TTGTATTGAGTTAAACATTTTTT-ACCG-5 ' 11-20 13-20 15-20 17-20 19-20 M 9-20 5 ' -AUAACUCAAUUUGUAAAAAA-GUUUU. . .-3. crRNA-sp2 C kbf mismatched targets protospacer 2 10 9-20 5 ' -TATTGAGTTAAAGTAAAAAA-3 ' non-complementary 5' -TTATATGAACATAACTCAATTTGTAAAAAAGGGTATTGGGGAATTCATTA-3 ' target DNA -~ 2640 bp IIIIIIIII 3 11-20 5 ' -TATTGAGTTATTGTAAAAAA-3 ' complementary 3' -AATATACTTGTATTGAGTTAAACATTTTTTCCCATAACCCCTTAAGTAAT-5' ~ 1880 bp 13-20 5 ' -TATTGAGTATTTGTAAAAAA-3' 15 5 ' -AUAACUCAAUUUGUAAAAAAGUUUUAGAGCUAUGCUGUUUUG-3 ' crRNA-sp2 15-20 5 ' -TATTGACAATTTGTAAAAAA-3 'Step by Step Solution
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