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In the mass spectrometer, the proteins must first be ionized. One way to do this is to shine a laser beam at the proteins, which
In the mass spectrometer, the proteins must first be ionized. One way to do this is to shine a laser beam at the proteins, which can remove an electron from each protein. We will assume each protein is singly ionized, which means each protein has a charge of +e : +1.60 X 10'19 C. The ionized proteins are then accelerated in a vacuum chamber by a uniform electric field. We will take the field to be 240,000 WC and the distance through which the proteins are accelerated to be 5.00 cm. After being accelerated through that distance of5.00 cm, the proteins then enter a 1.50meter long flight tu be, where they travel at constant velocity because there is no electric field. Proteins of different masses travel through the flight tube at different speeds, and so take different times to travel the length ofthe flight tu be. By measuring the time ta ken to traverse the flight tu be (the time-offlight), the mass can be determined and the specific protein identified. Protein masses are specified in atomic mass units (sometimes referred to as daltons). 1 atomic mass unit: 1.66 x 10'27 kg. Part (a) Homework . Unanswered . Take the mass of one specific protein to be 2090 atomic mass units. Calculate the magnitude of the protein's acceleration (remember that it is singly ionized) in the uniform field E = 240000 N/C. m/s2 Type your numeric answer and submit Unanswered . 5 attempts left Submit Part (b) Homework . Unanswered . Assume the protein started from rest, calculate the time it takes to cover 5.00 cm with the acceleration you calculated in part (a). Sa Part (c) _ O Homework ' Unanswered ' c. Continuing from part (b), calculate the speed the protein has after it has accelerated through the 5.00 cm distance. m/s Type your numeric answer and submit Unanswered o 5 attempts left @ Part (d) __ O Homework 0 Unanswered - The speed you found in part (c) is the constant speed the protein travels at along the 1.50-m distance of the flight tube. The mass spectrometer measures the time it takes the protein to cover this distance. Calculate that time. A second protein has a mass of 2120 atomic mass units. Calculate the difference between the time it takes the original protein to travel the 1.50 meters of the flight tube and the time it takes this second protein to do so. This will give you an idea of what the time resolution of the mass spectrometer has to be to distinguish between these two proteins. S
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