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In the small - scale pilot plant the yeast Saccharomyces cerevisiae is produced in fed - batch cultivations, to test new feeding strategies. The process
In the smallscale pilot plant the yeast Saccharomyces cerevisiae is produced in
fedbatch cultivations, to test new feeding strategies. The process consists of two
phases: an initial batch phase and a subsequent fedbatch phase. In order to
maximise the biomass yield, the substrate concentration during the fedbatch phase
shall be kept close to Design an appropriate feeding strategy starting at
process time using the data from the batch phase below.
Initial volume of the medium in the ideally stirred tank reactor:
The process can be described with the following mathematical model:
fedbatch operation
With Substrate conc. in inlet feed solution, Inlet flow rate, Volume of
medium in reactor, Biomass conc., specific substrate uptake rate,
maximum specific substrate uptake rate, Substrate affinity constant,
Substrate conc. in reactor, specific biomass formation rate, Yield
coefficient of biomass on substrate, Outlet flow rate.
a Plot using the above data the time course of the batch phase of the cultivation in an
appropriate graph.
b Calculate the overall yield coefficient and the specific biomass formation rate
as a function of time.
c Calculate the specific substrate uptake rate at and estimate the
maximum specific substrate uptake rate if
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