In this experiment, you find $3$ peaks that elute in the collected volumes of $4$ ml$,10$ ml$,$ and $14$ ml$.$

a$.$ What is Vo $($in ml$)?$

b$.$ What is the value of Kav for cytochrome c$?$

c$.$ The last volume you can see color is when $16$ ml are collected. Assume you are following the instructions and collecting in $1$ ml aliquots, what is the MINIMUM number of tubes you should have collected?