It is assumed that 10.00mL TRIS sample (TRIS; tris-(hydroxymethyl)aminomethane, (HOCH2)3CNH2 ) with a concentration of 24g TRIS per L sample is titrated with 0.100M hydrochloric acid. Adding HCl solution to the TRIS sample in excess of the equivalence point will influence the results of the concentration determination. 4. Calculate the determined concentration of the TRIS sample if 1 drop, 50L, of HCl solution has been added in excess of the equivalence point. Calculate the pH value in the sample if it has been added 1 drop less than that required to reach the equivalence point. pKa=8.06 for TRIS