lipase sample has total units $100$KU and specifc activity $45000$u$/$mg of protein, sample weight is $2\mathrm{.}22$mg$.$ i need enzyme activity. vender method used here to calculate specific activity is Procedure

Equilbrate with stirring $15$ ml of substrate $25$C $\backslash$pm $0\mathrm{.}2$C for Lipase, EPC No$.$L$397,$ and EPC No$.$HL$938,$ and to $37\backslash$deg C $\backslash$pm $0\mathrm{.}3$C for Lipase EPC No$.$LC$452$ and EPC No$.$IC$398.$ Titrate to a steady pH $8\mathrm{.}5$ with $0\mathrm{.}1$ M NaOH. Add $0\mathrm{.}002$ to $0\mathrm{.}004$ mg of enzyme. When the pH reaches $8\mathrm{.}0$ start a timer and deliver titrant $(0\mathrm{.}0100$ M NaOH$)$ having the outlet from the buret ca$.5$ mm below the surface of the stirring reaction mixture. $($It is convenient to use silicone tubing from the buret tip to reaction mixture.$)$ Maintain a constant pH $8\mathrm{.}0$ for $2$ to $4$ minutes and record the sample volume of titrant delivered per minute. Determine a blank rate by repeating the titrant in procedure on $15\mathrm{.}0$ ml of substrate without added enzyme. The blank volume of titrant should be less than $0\mathrm{.}10$ ml during $2$ to $4$ minutes. Record the blank volume of titrant delivered per minute.

Calculation of Specific Activity

units $/$mg$=(($Sample Vol$-$Blank Vol$)$ x $0\mathrm{.}01200$ x $1000)/$mg Enzyme