Lisa is running an experiment to determine the kinetic parameters (Km and Vmax) of the enzyme that she is studying. She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below. Substrate Concentration (mm) Velocity (MM/sec) 2.5 1 5 10 20 Nola 6.3 7.6 9 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax