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Liver Enzyme Lab - Part 1 name: (16 marks - 1 for hypothesis, 10 for table, 4 for conclusion) Purpose: to observe the how
Liver Enzyme Lab - Part 1 name: (16 marks - 1 for hypothesis, 10 for table, 4 for conclusion) Purpose: to observe the how the enzyme catalase reacts with hydrogen peroxide Hypothesis: Materials: test tubes, safety glasses, liver, hydrogen peroxide, water Procedure: Observe eoch of the test tube reoctions in the Enzyme Lab procedure and fill in the data table below. Observations: Test Contents Observations Interpretations Tube # 3 4 6. Conclusion: (In this area simply state what you learned obout the enzyme catalase) Enzyme Liver Lab Part 2 Using the above lab as an example, design an experiment in which you will choose one variable to change. Use the decomposition reaction of hydrogen peroxide catalyzed by the catalase enzyme in liver to design an experiment. This experiment will investigate either the effect of pH, temperature, substrate concentration, or a competitive inhibitor on the rate of this enzyme catalyzed reaction. Remember that a good experiment will test only one variable while all the other variables are controlled. Your lab will follow the formal lab write up criteria. Be sure to include a purpose which is a clear statement of the question (your problem), a hypothesis (what you think should happen - not necessarily what does), a materials list of any reagents (liver, hydrogen peroxide) and/or equipment you will use (safety glasses), a description of the possible procedure (step by step instructions) including a suitable control, and an explanation of how you would report your findings to your peers. Would it be a data table? Whatever variable you choose for your experiment be sure to use a full range of tests. For exomple, if I were to test the effects of temperature I would run the experiment at 5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 degrees Celsius. If I was going to test is pH affects the reaction, I would ensure a range of pH levels would be encountered. Remember that is a design for an experiment - you are NOT actually doing the experiment. Worth 15 morks: Title Purpose Hypothesis /1 Materials /2 Procedure /5 Proper Use of a Control /2 Data Reporting 13 /1 /1
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