- Make a graph of the above-mentioned data and compare the data to regression fits of the following virial expression for the osmotic pressure, i.e., = (RTc2/MW2)[1+Bc2+Cc22].

- Show that when B and C are equal to zero, the resulting expression, i.e., vant Hoffs law, fits the data quite well at low hemoglobin concentrations where the solution is expected to be ideal.

- Next, explore how the fit to these data improves by performing a nonlinear regression to find the value of B, with C = 0.

- Then, perform another nonlinear regression that includes B and C.

2.29 The following data for the osmotic pressure of hemoglobin (MW=68000g/mol) in an aqueous solution at 0C is presented by Freeman (1995)