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Mass balances and rates in a fermenter (15 points). In a batch fermentation with yeast (X) using substrate (S), a valuable pharmaceutical product (P) is

Mass balances and rates in a fermenter (15 points). In a batch fermentation with yeast (X) using substrate (S), a valuable pharmaceutical product (P) is produced. The volume of the culture is constant at 250 L. The experimental data for your process is shown below:

t (h)

S (g/L)

X (g/L)

P (g/L)

0.0

50.0

0.13

0.00

0.2

50.0

0.13

0.00

0.4

50.0

0.13

0.00

0.6

50.0

0.13

0.00

0.8

50.0

0.17

0.00

1.0

49.9

0.21

0.00

1.2

49.9

0.27

0.00

1.4

49.8

0.34

0.01

1.6

49.8

0.43

0.01

1.8

49.7

0.55

0.01

2.0

49.6

0.70

0.01

2.2

49.4

0.89

0.02

2.4

49.3

1.13

0.02

2.6

49.0

1.43

0.03

2.8

48.7

1.82

0.04

3.0

48.4

2.32

0.05

*Units for X are in grams dry cell weight per L.

(8) In the region of exponential growth, calculate the following specific rates: mmax, qsmax , and qpmax .

(2) In the region of exponential growth, what is the yield of grams P produced per grams S consumed?

(3) At t=5h, what are the concentrations of X and P in the fermenter?

(2) Suppose you scale up the process from 250 L to 1,000 L. The 1,000 L is operated at the same process conditions (temperature, pH, initial concentrations, etc.) as the 250 L process. At the 1,000 L scale, what is qS? At the 1,000 L scale, how many grams of S are in the fermenter at t=5h?

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