Question
Mass balances and rates in a fermenter (15 points). In a batch fermentation with yeast (X) using substrate (S), a valuable pharmaceutical product (P) is
Mass balances and rates in a fermenter (15 points). In a batch fermentation with yeast (X) using substrate (S), a valuable pharmaceutical product (P) is produced. The volume of the culture is constant at 250 L. The experimental data for your process is shown below:
t (h) | S (g/L) | X (g/L) | P (g/L) |
0.0 | 50.0 | 0.13 | 0.00 |
0.2 | 50.0 | 0.13 | 0.00 |
0.4 | 50.0 | 0.13 | 0.00 |
0.6 | 50.0 | 0.13 | 0.00 |
0.8 | 50.0 | 0.17 | 0.00 |
1.0 | 49.9 | 0.21 | 0.00 |
1.2 | 49.9 | 0.27 | 0.00 |
1.4 | 49.8 | 0.34 | 0.01 |
1.6 | 49.8 | 0.43 | 0.01 |
1.8 | 49.7 | 0.55 | 0.01 |
2.0 | 49.6 | 0.70 | 0.01 |
2.2 | 49.4 | 0.89 | 0.02 |
2.4 | 49.3 | 1.13 | 0.02 |
2.6 | 49.0 | 1.43 | 0.03 |
2.8 | 48.7 | 1.82 | 0.04 |
3.0 | 48.4 | 2.32 | 0.05 |
*Units for X are in grams dry cell weight per L.
(8) In the region of exponential growth, calculate the following specific rates: mmax, qsmax , and qpmax .
(2) In the region of exponential growth, what is the yield of grams P produced per grams S consumed?
(3) At t=5h, what are the concentrations of X and P in the fermenter?
(2) Suppose you scale up the process from 250 L to 1,000 L. The 1,000 L is operated at the same process conditions (temperature, pH, initial concentrations, etc.) as the 250 L process. At the 1,000 L scale, what is qS? At the 1,000 L scale, how many grams of S are in the fermenter at t=5h?
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