Match each chromatographic method with the characteristics of the protein that you would expect to find in the flow-through or in early fractions. Cation-exchange chromatography Anion-exchange chromatography Choose Low molecular weight proteins Negatively-charged proteins Hydrophobic proteins Untagged proteins Hydrophilic proteins High molecular weight proteins Positively-charged proteins Ex-His tagged proteins Size-exclusion chromatography Hydrophobic interaction chromatography [Choose) Immobilized metal ion affinity chromatography (IMAC) [Choose In a standard enzymatic reaction (A), you add 0.46 mg of enzyme (MW 59,800) to catalyze a reaction according to the following curve: 3- A 2- Reaction rate (mol substrate min) 1- AB 4 2 8 10 Time (s) a. What is the approximate kcat of the reaction in (A)? b. The reaction in (B) is done with the same amount of a different enzyme. What is the difference in kcat between the two reactions? a