Miles was performing a noncompetitive immunoassay for IgG subclasses and obtained the following results:

IgG $1490$ mg$/$dL

G$1713$ mg$/$dL

G$2101$ mg$/$dL

G$360\mathrm{.}7$ mg$/$dL

G$430\mathrm{.}7$ mg$/$dL

The calculated sum of the $4$ IgG subclasses is $905\mathrm{.}4$mg$/$dL but the total measured IgG was $1490$mg$/$dL which is a differnce of $584\mathrm{.}6$mg$/$dL$.$

Miles begins troubleshooting, starting his investigation into the discrepant results by looking at the raw data from the analyzer, which is shown here:

Raw Data Dilution Result

IgG $1$:$4001490$ mg$/$dL

$1$:$2000(1450$ mg$/$dL$)$

G$11$:$100713$ mg$/$dL

$1$:$400(<1120$ mg$/$dL$)$

G$21$:$20101$ mg$/$dL

$1$:$100(<325$ mg$/$dL$)$

G$31$:$10060\mathrm{.}7$ mg$/$dL

$1$:$400(56\mathrm{.}3$ mg$/$dL$)$

G$41$:$10030\mathrm{.}7$ mg$/$dL

$1$:$400(>242$ mg$/$dL$)$

$1$:$2000737$ mg$/$dL

$1$:$8000(702$ mg$/$dL$)$

$3.$ Do the total IgG dilutions match within $10\%?$

$4.$ Do the IgG$4$ dilutions match within $10\%?$

$5.$ What is a possible explanation for these results?