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MR_VP 1-Why is it important to mix the VP test tube after reagents are added? 2-Why is the methyl red test read right after reagents

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1-Why is it important to mix the VP test tube after reagents are added?

2-Why is the methyl red test read right after reagents are added, while the Voges-Proskauer results are not?

3-Some microbiologists recommend to re-incubate organisms producing methyl-red negative results for an additional 48 hours. Why would this be done?

4-If the methyl red test results show a color change to red but the Voges-Proskauer test shows no color change what does this indicate?

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