narrow-pore columns (50 mm instead of 100 or 150 mm) packed with 1.7 um particles. However, under certain chromatographic conditions, the opposite results might be observed (i.e., much worse resolution, and less efficient separation). Figure Q07 below shows two separation experiments with a significan decrease in Rs (decreased by a factor of 5 "from 3.0 to 0.3) when the narrow-pore, short column packed with 1.7um particles was used. Could you please explain this observation? 74 dininkenni the 150 x 4.6 mm Sum 24 mumin (1950 psi) 90% ACN / 12.5 min 24 ore 24-ured alli tih creates 24 horpe orghe 50 x 21 mm/2m 0.25 mL/min (2035 psi) 20 - 90% ACN / 12.5 min R 0.6 4. 10 1214 18 Time narrow-pore columns (50 mm instead of 100 or 150 mm) packed with 1.7 um particles. However, under certain chromatographic conditions, the opposite results might be observed (i.e., much worse resolution, and less efficient separation). Figure Q07 below shows two separation experiments with a significan decrease in Rs (decreased by a factor of 5 "from 3.0 to 0.3) when the narrow-pore, short column packed with 1.7um particles was used. Could you please explain this observation? 74 dininkenni the 150 x 4.6 mm Sum 24 mumin (1950 psi) 90% ACN / 12.5 min 24 ore 24-ured alli tih creates 24 horpe orghe 50 x 21 mm/2m 0.25 mL/min (2035 psi) 20 - 90% ACN / 12.5 min R 0.6 4. 10 1214 18 Time