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Need compelet answer votup 2. Phosphorus in urine can be determined by treating with molybdenum(VI) and then reducing the phosphomolybdate with aminonaphtholsulfonic acid to give
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2. Phosphorus in urine can be determined by treating with molybdenum(VI) and then reducing the phosphomolybdate with aminonaphtholsulfonic acid to give the characteristic molybdenum blue color. This absorbs at 690nm. A patient excreted 1270mL urine in 24h, and the pH of the urine was 6.5. A 1.00mL aliquot of the urine was treated with molybdate reagent and aminonaphtholsulfonic acid and was diluted to a volume of 50.0mL. A series of phosphate standards was similarly treated. The absorbance of the solutions at 690nm, measured against a blank, were as follows: a. Calculate the number of grams of phosphorus excreted per day. b. Calculate the phosphate concentration in the urine as millimoles per liter. 3. A recent graduate of Uni MAP was fortunate enough to obtain a job at a new start-up company in KL. This company's first product was going to be a small portable spectrophotometric meter and single-use disposable that would be able to detect the levels of total hemoglobin ( Hb ) in a single drop of whole blood (obtained by finger prick), very similar to a very successful product already on the market. The UniMAP grad was assigned the task of determining the optimal path-length specification that would be required in the disposable plastic cuvette into which the drop of blood would wick (to be manufactured by injection molding). The student knew the following: a) the normal range of Hb in blood is 1119g/dl (grams per 100ml); b) Hb has a molecular weight of 43,500 (on average); c) the molar absorptivity of cyanmethemoglobin (the species that would be formed by the dry reagents in the cuvette) is 25,000M1cm1 at 540nm; d) the proposed instrument would have an LED light source centered at this wavelength; and e) the most precise absorbance measurements can be made when the measured absorbance value is close to 0.5. Hence, since the student had taken analytical chemistry subject, he/she decided that it would be best if the cuvette were designed to yield this absorbance value (and thus optimal precision) at the average normal concentration of Hb in blood. What pathlength in millimeters (mm) should this former student propose to the engineers designing the disposable cuvetteStep by Step Solution
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