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3.) On the next page is the result of a Beers' Law plot obtained in the lab. As can be seen, the Y-intercept is


3.) On the next page is the result of a Beers' Law plot obtained in the lab. As can be seen, the Y-intercept is below zero, when Beer's Law indicates that it should be 0. Upon investigation, it was determined that the student used a common test tube instead of a cuvette to calibrate the spectrophotometer. He then used a cuvette for measuring his samples. a. Explain why this produced a lowering of the line. 0.95 0.9 0.85 0.8 0.75 0.7 0.65 0.6 0.55 0.5 0.45 0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 - 0.05 - 0.1 - 0.15 -0.2 0. 14 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Concentration (M x 10-5) What will be the effect on the calculated concentration of the unknowns?

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