Part II: 02 and CO2 transfer (Tn, 0 \& Tn,c) For a 1,3 Propanediol (C3H8O2) - PDO - production organism (C1H1.8O0.5N0.2) a first chemostat experiment is performed and measurements are made when the chemostat is at steady state. PDO is produced aerobically using glucose (C6H12O6) as a substrate and NH4+as N-source. The chemostat feed, Fin in m3/h, contains glucose and (NH4)2SO4 dissolved in water. Air sparging, with rate FN,in in mol/h, is used to supply O2 to the organism and to remove CO2. The inflow of the dry sparged air contains much more CO2 than normal and its composition is 21%O2;0.5%CO2 and 78.5%N2. Question 1 0.0/1.0 point (graded) First FN,out must be calculated, which balance do you use for this? broth O2-balance gas N2-balance gas O2-balance 0.0/1.0 point (graded) Calculate FN,out (assuming the solubility of N2 in the broth is negligible); give the correct units. 1 point possible (graded) TN,O is the rate of transfer of O2 from the gas phase to the liquid phase. Which balance do you use to calculate TN,O broth O2-balance gas CO2-balance gas O2-balance 2 points possible (graded) Calculate TN,O; give the correct units. 2 points possible (graded) TN,C is the rate of transfer of CO2 from the liquid phase to the gas phase. Calculate TN,c; give the correct units