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Penicillin F is recovered from a dilute aqueous fermentation broth by extraction with amyl acetate, using 6 volumes of solvent per 1 0 0 volumes

Penicillin F is recovered from a dilute aqueous fermentation broth by extraction with amyl acetate, using 6 volumes of solvent per 100 volumes of the aqueous phase. At pH =3.2 the distribution coefficient Kd is 80.
1. What fraction of the penicillin would be recovered in a single ideal stage? 2. What would be the recovery with two-stage extraction using fresh solvent in both stages?

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