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PLEASE ANSWER ASAP WILL GIVE THUMBS UP A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: -amylase, 200kDa
PLEASE ANSWER ASAP WILL GIVE THUMBS UP
A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: -amylase, 200kDa with elution volume 10.5mL Aprotinin, 6.5kDa with elution volume 39.7mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 34.7. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Please don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28). YourStep by Step Solution
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