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Please answer question one part a and b 1. Tris (Tris[hydroxymethyl]aminomethane) is a commonly used buffer with a pKa= 8.06. In making up a Tris
Please answer question one part a and b
1. Tris (Tris[hydroxymethyl]aminomethane) is a commonly used buffer with a pKa= 8.06. In making up a Tris solution, an appropriate amount of the free base is dissolved in water and the pH of the solution is adjusted, often with HCl. The molecular weight of Tris is 121.1. (a) Describe the preparation of 1L of 25mM solution, pH=8.4, using solid Tris base and 1MHCl. (b) Draw the structure of Tris in its protonated and unprotonated formsStep by Step Solution
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