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Please answer the following questions using the given information. an amino acid sequence of a heptapeptide gave the following information: Asp, Ala, Lys , Met,
Please answer the following questions using the given information.
an amino acid sequence of a heptapeptide gave the following information: Asp, Ala, Lys Met, Phe, and Tyr
Trypsin had no effect
PITC analysis gave the phenyl alanine derivative
Chymotrypsin which cleaves after aromatic amino acids gave a dipeptide and tetrapeptide, which contained Leu, Lys and Met.
Cyanogen bromide, which cleaves after methionine residues, gave a dipeptide, tetrapeptide, and free lys
Question Trypsin had no effect on the polypeptide what does this tell you about the sequence N and C terminus?
Question PITC analysis gave the phenyl alanine derivative what does this tell you about the N and C terminus?
Question please show the correct cleavages of each step
Question Using the information above what is the new final sequence?
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