Please ensure NO PLAGIARISM can be detected.

Answer question 1 (a) and (b) giving a detailed answer for each part.

When conducting an experiment to examine the catalytic activities of three enzyme mutants (E97Q, E97D, E165A) the following Kcat values were obtained E97Q = 1.005131663, E97D = 42.42116401, and E165A = 0.444048421

(a)making a reference to the kcat values obtained, write about the importance of the mutated residues at both positions 97 and 165. In your answer make sure you ALSO include to compare the kcat values, active sites and much more.

(b) speculate on the roles of these 2 residues in the catalytic mechanism. your answer should ALSO mention the polarities, shape, catalytic activity and what the kcat values mean in reference to this.