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Please give the workflow for the below question: You have single cell sequence ( sc seq ) data in both cell ranger files and fastq.
Please give the workflow for the below question:
You have single cell sequence sc seq data in both cell ranger files and fastq. You can start from either.
There are pipelines that do QC trimming, alignment, etc.
Cell ranger by x also does these steps. These cell rangers generated files or the ones from other pipelines are then used to make UMAPs and DEGs lists, etc.
The things you need to eventually do are
Compare groups to each other, Compare clusters to other clusters. and generate gene lists, pathways etc from those comparisons.
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