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15.46 The hydrolysis of N-glutaryl-L-phenylalanine- p-nitroanilide (GPNA) to p-nitroaniline and N-glutaryl-L-phenylalanine is catalyzed by -chymotrypsin. The following data are obtained: where [S] = GPNA. Assuming Michaelis-Menten kinetics, calculate the values of Vmax,KM, and k2 using the Lineweaver-Burk plot. Another way to treat the data is to plot v0 versus Problems 737 v0/[S], which is the Eadie-Hofstee plot. Calculate the values of Vmax,KM, and k2 from the Eadie-Hofstee treatment, given that [E]0=4.0106 M. [Source: J. A. Hurlbut, T. N. Ball, H. C. Pound, and J. L. Graves, J. Chem. Educ. 50, 149 (1973).]