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please show working and the graph plotted Protein concentration can be determined spectrophotometrically using the Bradford assay. Solutions of varying standard protein concentrations are mixed
please show working and the graph plotted
Protein concentration can be determined spectrophotometrically using the Bradford assay. Solutions of varying standard protein concentrations are mixed with reagents that cause the development of a color. The amount of color produced depends on the amount of protein present. The absorbance measurement at 595nm is directly proportional to the [Protein]. By plotting the [Protein] vs Absorbance (calibration curve) the concentration of an unknown protein also treated similarly with the Bradford Reagent can be determined. Using the data below calculate the unknown Concentrations in mg/ml : 0.2ml Unknown Protein Soln 0.22 Step by Step Solution
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