pls answer all 3

You have a mixture of 4 amino acids (Q,W,E,R,T) that you want to separate using ion exchange chromatography. Which of these amino acids would bind most strongly to a CM cation exchange column at pH 7? Q W E R T Which of the followIng methods would be expected to help elute (I.e., WEAKEN the binding of) proteins from a CATION EXCHANGE column with carboxymethyl groups in the stationary phase and a mobile phase with pH=6.0. Each correctly assigned choice (i.e., either checked or not) earns 0.25 marks. Each incorrectly assigned choice earns 0 marks. Increasing the concentration of salt (NaCl) in the mobile phase from 0.1M to 0.5 M for a protein with pl=7.0. Decreasing the pH of the mobile phase from 6.0 to 5.0 for a protein with pl= 6.5 Increasing the pH of the mobile phase from 6.0 to 7.0 for a protein with pl=6.5. Increasing the pH of the mobile phase from 6.0 to 7.0AND increasing the concentration of salt (NaCl) from 0.1M to 0.5M for a protein with pl=7.5. Use the 1-letter code for amino acids to name the peptide above. Make sure to pay attention to the normal conventions for capitalization and polarity of peptide sequences (i.e., sequences always start from the N-terminus of the peptide). BE VERY CAREFUL -- NO PART MARKS ARE GIVEN FOR THIS QUESTION. The ENTIRE sequence must be correct to earn a mark for this