Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

pls explain You will use DLS to characterize the size of your synthesized liposomes. In this lab, you use PC and PE to produce large

pls explain
image text in transcribed
You will use DLS to characterize the size of your synthesized liposomes. In this lab, you use PC and PE to produce large multilamellar vesicles (MLVs), freeze/thaw them to produce smaller liposomes, and then extrude them through a 200 nm pore filter to form large unilamellar vesicles (LUVS). You will use DLS before and after extruding to determine the size and polydispersity (breadth of distribution of sizes). The polydispersity is defined in DLS as the ratio of: (standard deviation of the diameter measurements) / (average diameter). Suppose you get two measurements: A: diameter - 350 nm; PDI -0.014 B: diameter - 947 nm; PDI -0.216 What would you conclude from the data? liposomes in sample A are smaller than in B, and mostly all the same size o liposomes in sample B are smaller than in A, and mostly all the same size O liposomes in sample A are smaller than in B, but have wildly varying sizes o liposomes in sample B are smaller than in A, but have wildly varying sizes

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Organic Chemistry

Authors: John McMurry

5th Edition

534362745, 978-0534362744

More Books

Students also viewed these Chemistry questions

Question

What would be the funniest superpower?

Answered: 1 week ago