POST-LAB QUESTIONS 1. Calculate the enzyme activities with acetylcholine as the substrate for both enzymes. Using the change in absorbencies between 30sec, and 2min, calculate the rate of reaction as change () in absorbance/min. for a. acetylcholine esterase b. earboxyl esterase c. You have calculated the amount of acetylcholine esterase that you had in the sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase that you had in the sample tube (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. e. Which enzyme catalyzes the hydrolysis of acetylcholine faster? How many times faster? 2. Calculate the enzyme activities of o-nitrophenyl acetate as a substrate for both enzymes. Using the change in absorbence between 30sec and 2min. in the reaction, calculate the rate of reaction toward o-nitrophenyl acetate in absorbence/min. for a. acetylcholine esterase b. carboxyl esterase c. You have calculated the amount of acetylcholine esterase in your sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase in your sample tabe (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. 6. Which enzyme catalyzes the hydrolysis of o-nitrophenyl faster? How many times faster? POST-LAB QUESTIONS 1. Calculate the enzyme activities with acetylcholine as the substrate for both enzymes. Using the change in absorbencies between 30sec, and 2min, calculate the rate of reaction as change () in absorbance/min. for a. acetylcholine esterase b. earboxyl esterase c. You have calculated the amount of acetylcholine esterase that you had in the sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase that you had in the sample tube (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward acetylcholine in absorbence/min/mg enzyme. e. Which enzyme catalyzes the hydrolysis of acetylcholine faster? How many times faster? 2. Calculate the enzyme activities of o-nitrophenyl acetate as a substrate for both enzymes. Using the change in absorbence between 30sec and 2min. in the reaction, calculate the rate of reaction toward o-nitrophenyl acetate in absorbence/min. for a. acetylcholine esterase b. carboxyl esterase c. You have calculated the amount of acetylcholine esterase in your sample tube (Pre-Lab Question no. 3). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. d. You have calculated the amount of carboxyl esterase in your sample tabe (Pre-Lab Question no. 4). Use this number to calculate the activity of this enzyme toward o-nitrophenyl acetate in absorbence/min/mg enzyme. 6. Which enzyme catalyzes the hydrolysis of o-nitrophenyl faster? How many times faster
