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Prepare 1 0 0 m L of 1 M Tris - H C l , p H 7 . 4 , by adding 0 .

Prepare 100mL of 1M Tris-HCl,pH7.4, by adding 0.1 moles (12.1g) of Tris base to 80mL of ddH2O, and then adding 12.5mL of conc. HCl(6M). Measure the pH of a 1:20 dilution of 1M stock (0.2 mL diluted to 4mL
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