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Problem 1: In the process of fluorescence, a molecule in its ground state will absorb a photon with a certain energy Eex, called the excitation
Problem 1: In the process of fluorescence, a molecule in its ground state will absorb a photon with a certain energy Eex, called the excitation energy, and then emit a photon with energy Eem, the emission energy. Obviously, the molecule cannot emit more energy than was absorbed. a) You wrote in your notebook that you excited a fluorescent bead at 2 = 640 nm and found that it fluoresced (emitted) at 2 = 520 nm. Or was it the other way around? Do a calculation and see if those figures should be switched. b) You shine a laser on your bead at the excitation wavelength. The laser has a power of 1 mW. How many photons are emitted by the laser in one second
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