Problem 2 (4 pts) The F1 & R1 primers are used to PCR amplify a DNA fragment from the template shown below. F1 Forward Primer: 5' GCCAGTAG 3' R1 Reverse Primer: 5' GGGTCC 3' 5' ATAGCCAGTAGTACCATTGATACGTGACCATACGTCGGACATAGTTGGACCCAGTACAGTTT 3' a) Indicate where the forward and reverse primers bind to the template. b) How long is the amplified PCR product (don't forget to include units)? Problem 3 (2 pts) Choose two primers that can be used to PCR-amplify the bold portion of the DNA segment shown below (no more and no less). All primer sequences are in the 5'--> 3 direction. 5'-ATGTGTGATCTAAACGTAGCCGGATACGTATACTCGCATTAGTAAACTGT-3' 3'-TACACACTAGATTTGCATCGGCCTATGCATATGAGCGTAATCATTTGACA-5' primer #1: AAATCTA primer #2: ATCTAAA primer #3: GTAATCA primer #4: TGATTAC primer #5: ACTAATG primer #6: CATTAGT primer #7: TAGATTT primer #8: TTTAGAT primer #9: ATGTGTG primer #10: GTGTGTA primer #11: TACACAC primer #12: CACACAT primer #13: AAACTGT primer #14: TGTCAAA primer #15: TTTGACA primer #16: ACAGTTT Problem 2 (4 pts) The F1 & R1 primers are used to PCR amplify a DNA fragment from the template shown below. F1 Forward Primer: 5' GCCAGTAG 3' R1 Reverse Primer: 5' GGGTCC 3' 5' ATAGCCAGTAGTACCATTGATACGTGACCATACGTCGGACATAGTTGGACCCAGTACAGTTT 3' a) Indicate where the forward and reverse primers bind to the template. b) How long is the amplified PCR product (don't forget to include units)? Problem 3 (2 pts) Choose two primers that can be used to PCR-amplify the bold portion of the DNA segment shown below (no more and no less). All primer sequences are in the 5'--> 3 direction. 5'-ATGTGTGATCTAAACGTAGCCGGATACGTATACTCGCATTAGTAAACTGT-3' 3'-TACACACTAGATTTGCATCGGCCTATGCATATGAGCGTAATCATTTGACA-5' primer #1: AAATCTA primer #2: ATCTAAA primer #3: GTAATCA primer #4: TGATTAC primer #5: ACTAATG primer #6: CATTAGT primer #7: TAGATTT primer #8: TTTAGAT primer #9: ATGTGTG primer #10: GTGTGTA primer #11: TACACAC primer #12: CACACAT primer #13: AAACTGT primer #14: TGTCAAA primer #15: TTTGACA primer #16: ACAGTTT