Problem 2. A mixture of albumen and lysozyme is separated by a column chromatography with a 1m length, which is filled with 500 grams of resins. The resin having a 1.2g/cm3 density and 0.5 fractional participle porosity (Ep=0.5). Assume the resin has cylindrical pores and an average pore diameter is 25A(1010m). Albumin peak is detected after 5,000 seconds having a width of 600 seconds and a 0.3 absorbance peak ( Cmax). Lysozyme peak is detected after 5,500 seconds having a width of 800 seconds and a 0.8 absorbance peak (Cmax). Both peaks follow Gaussian distribution with standard deviations. 2.1. What were the individual velocities of albumen and lysozyme? 2.2. What is the resolution? 2.3. What is the mass fraction of albumin in the feed mixture? (Assume there exist only albumin and lysozyme proteins in the feed.) Problem 2. A mixture of albumen and lysozyme is separated by a column chromatography with a 1m length, which is filled with 500 grams of resins. The resin having a 1.2g/cm3 density and 0.5 fractional participle porosity (Ep=0.5). Assume the resin has cylindrical pores and an average pore diameter is 25A(1010m). Albumin peak is detected after 5,000 seconds having a width of 600 seconds and a 0.3 absorbance peak ( Cmax). Lysozyme peak is detected after 5,500 seconds having a width of 800 seconds and a 0.8 absorbance peak (Cmax). Both peaks follow Gaussian distribution with standard deviations. 2.1. What were the individual velocities of albumen and lysozyme? 2.2. What is the resolution? 2.3. What is the mass fraction of albumin in the feed mixture? (Assume there exist only albumin and lysozyme proteins in the feed.)