Protein synthesis requires a lot of energy, most of which is in the form of ATP or GTP. Generally, energy is required at steps where fidelity of peptide synthesis is involved. Which of the following components of the protein synthesis machinery uses ATP or GTP to assure production of proper proteins? Answers AE A site B IF1 C eEF2 D. 16S rRNA E 18 I rRA With which of the following restriction endonucleases you would cut pBR322 in order to insert human DNA that has been cut with Bgill? Hint: Bgill cuts the sequence AlGATCT at the arrow. Answers A - E A Xhol ClTCGAG B HaellI GGjCC C Hhal GCGC D ECORI GAATTC E BamHI GIGATCC Which of the following statements applies to prokaryotic, but NOT eukaryotic transcription? Answers A - E A Transcription termination is not exact B mRNA cleavage precedes polyadenylation C Genes for structural RNAs (tRNA, rRNA) are polycistronic D Translation begins before transcription is completed E Transcription initially produces rRNA that must be processed for function